3D 배양잇몸모델을 이용한 알파-초산 토코페롤과 18베타-글리시레틴산의 잇몸 흡수 평가
Gingival absorption of α-tocopherol acetate and 18β-glycyrrhetinic acid : in vitro evaluationin reconstructed gingival tissue
김윤선, 김주애, 유아람, 조호성, 신재영, 이상화,
소속 상세정보
김윤선 ( Kim Yun-Sun ) - LG생활건강 기술연구원
김주애 ( Kim Ju-Ae ) - LG생활건강 기술연구원
유아람 ( You A-Ram ) - LG생활건강 기술연구원
조호성 ( Cho Ho-Song ) - LG생활건강 기술연구원
신재영 ( Shin Jae-Young ) - LG생활건강 기술연구원
이상화 ( Lee Sang-Hwa ) - LG생활건강 기술연구원
KMID : 0355420160400020079
Abstract
Objectives: To assess the absorption of α-tocopherol acetate and 18β-glycyrrhetinic acid, which are used as active ingredients in toothpaste, into a reconstructed gingival tissue.
Methods: EpiGingivalTM tissues were treated with a 25% slurry of toothpaste containing 2% α-tocopherol acetate and 0.3% 18β-glycyrrhetinic acid, for 2 minutes. The treatment was repeated up to 6 times, with 1 hour intervals. After completion of all treatments, the active ingredients in the tissue extracts and receiver solutions were measured by high performance liquid chromatography.
Results: Although α-tocopherol acetate was not detected, α-tocopherol was detected in the tissue extracts, indicating that α-tocopherol acetate was bioconverted to α-tocopherol after absorption. We could detect 18β-glycyrrhetinic acid both in the tissue extracts and in the receiver solutions, with a posi-tive correlation to the number of treatments.
Conclusions: We found that our toothpaste effectively delivered α-tocopherol acetate and 18β-glycyrrhetinic acid to a reconstructed gingival tissue in vitro.
키워드
Absorption; α-Tocopherol acetate; 18β-Glycyrrhetinic acid; Periodontal disease; Toothpaste
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